Figure 7From: Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?Smoke Preparation according to ISO/FTC protocol. Concentrated Smoke solution was prepared from each cigarette using CSM-SCSM Cigarette Smoking Machine according to ISO/FTC determination parameters. These require a puff volume of 35Â ml with duration of 2Â s at interval of 60s.Back to article page