Figure 6

Oxygen consumption rates (OCR) in transfected single muscle fibers. (A) Representative OCR (pMoles/min) for single muscle fibers isolated from flexor digitorum brevis (FDBs) of C57/BL6 wildtype male mice (3 mo). Solid black line (Contralateral control fibers) and dotted line (Transfected fibers). (B) Average OCR (pMoles/min) of single muscle fibers isolated from FDBs of C57/BL6 wildtype male mice (3 mo). Black bars (Contralateral control fibers) and white bars (Transfected fibers). Data are presented as the average OCR ± SE. N = 2 separate animals. Arrows represent successive additions of FCCP + pyruvate (400 nM + 10 mM respectively), and antimycin A (1 μM).