Figure 2

Genomic profiling of blood from developing HD monkeys. Total RNA from the peripheral blood was collected at 5, 11, 17, and 23 months and analyzed by Rhesus Macaque Affymetrix arrays. Histograms were generated from the array data. A) Probes with P-value and FDR ranges from less than 1.0E-07 to greater than 1.0E-01 obtained from 2-way ANOVA for the effect of disease are plotted. 2-way ANOVA analysis was from data spanning the 5, 11, 17, and 23 month samples. B) Probes with P-value and FDR ranges from less than 1.0E-07 to greater than 1.0E-01 obtained from 2-way ANOVA for the effect of age are plotted. C) Venn Diagram analysis of overlapping disease-associated mRNAs from the HD monkeys with results from human HD patient blood deposited into the NCBI/GEO database. The human HD blood samples were analyzed independently using two different array platforms (Affymetrix and Codelink) and compared with the rhesus HD-associated mRNAs by Venn Diagram. All analyzed mRNAs have a P value < 0.05. D) The expression values (Log2 transformed & normalized microarray probeset intensities) of 11 HD-associated mRNAs identified from the Rhesus profiling were median centered for heatmap analysis. Each column represent a single sample, and each row represents a single mRNA probeset. Green squares represent lower than median levels of mRNA expression; black squares represent median levels of mRNA expression; red squares represent higher than median levels of mRNA expression.