Figure 6

Coexposure to MEK and PKA inhibitors suppress DOM-stimulated BDNF overexpression and CREB phosphorylation. Organotypic slices were cultured in the presence of the MEK inhibitor PD98059 and the PKA inhibitor H89 for 1 h, and then treated with 2 μM DOM for 24 h (insult). Lysates were collected at the indicated hours post insult (HPI) and analyzed by Western blot using BDNF and β-actin antibodies (A) or p-CREB and total CREB antibodies (B). The blots correspond to representative experiments and values are the means ± SEM of at least four experiments performed from different cultures. (#P < 0.001 vs. None within the same group; *P < 0.001 vs None within C group).