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Figure 2 | BMC Neuroscience

Figure 2

From: New highly sensitive rodent and human tests for soluble amyloid precursor protein alpha quantification: preclinical and clinical applications in Alzheimer’s disease

Figure 2

Specificity of the assays. A: Recombinant proteins h-sAPPα and r-sAPPα purity. 0.5 μg of h-sAPPα and r-sAPPα separated in 10% SDS-PAGE and stained with Simply Blue Safe Stain. The purity of both recombinant proteins is shown by the absence of additional bands. B: Human sAPPα assay. Calibration curves were generated with various concentrations of the recombinant proteins h-sAPPα, h-sAPPβ, r-sAPPα and the commercially available human peptide h-Aβ1-42. Results were calculated from the 665 nm/620 nm ratios and expressed in delta F. Slope for h-sAPPα: 1.47 ± 0.05; slopes for other peptides: <0.05. C: Rodent sAPPα assay. Calibration curves were generated with various concentrations of the recombinant proteins r-sAPPα, h-sAPPβ, h-sAPPα and the commercially available peptide h-Aβ1-42. Results were calculated from the 665 nm/620 nm ratios and expressed in delta F. Slope for r-sAPPα: 0.73 ± 0.02; slope for h-sAPPα: 0.17 ± 0.01; slopes for other peptides: <0.05.

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BMC Neuroscience

ISSN: 1471-2202

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