Figure 1From: Selenium preserves mitochondrial function, stimulates mitochondrial biogenesis, and reduces infarct volume after focal cerebral ischemiaNeuroprotective effect of selenium pretreatment against glutamate toxicity and hypoxia. A. Effect of selenium pretreatment on glutamate toxicity and hypoxic cell death. Cell viability was measured 24 h or 10/12 h after glutamate and hypoxia/reoxygenation respectively. Selenium pretreatment significantly improved cell survival from glutamate toxicity (4 mM) and hypoxia/reoxygenation (10/12 h). B. Representative micrograph of HT22 cells exposed to glutamate (4 mM) and hypoxia/reoxygenation (10/12 h) with or without selenium pretreatment. Cell viability was calculated using MTT assay and relative cell viability was expressed as % of control set at 100%. Data represents means ± SD of 4 experiments conducted in triplicate. *P < 0.05, ***P < 0.001 vs. control and #p < 0.05, ###p < 0.001 vs. respective group. Se = selenium, Cont = control and Glu = glutamate.Back to article page