Figure 1From: Enhancement of musculocutaneous nerve reinnervation after vascular endothelial growth factor (VEGF) gene therapyImmunofluorescence staining for VEGF. Immunofluorescence staining for VEGF in the cryostat sections through intact musculocutaneous nerve (A) and distal stump of MCN transfected with plasmid phVEGF 2 months after its end-to-end (B) and end-to-side (C) neurorrhaphy. Double immunostaining for VEGF (Alexa-488) and neurofilaments (TRITC) indicated increased VEGF staining of Schwann cells in distal stump of MCN transfected with plasmid phVEGF 2 months after its end-to-end neurorrhaphy (D). Moreover, double immunostaining for VEGF (Alexa-488) and RECA (TRITC) displayed location of VEGF protein in blood vessels of transfected MCN stump 2 months after its end-to-side neurorrhaphy (E). Arrows - Schwann cells; arrowheads - blood vessels. Scale bars for A-C = 90 μm, D = 20 μm, E = 30 μm, for insets = 3 μmBack to article page