Figure 2

Pharmacology of CCh-induced increase in slope of neuronal APs firing. A. Plot of the reversibility (in %) of the CCh-induced increase in slope of neuronal APs firing after washout or addition of different mAChR antagonists to a CCh-containing ACSF as indicated. Note the reversibility observed with atropine (n = 7) or pirenzepine (n = 7) was maximal since not different to that observed after washout (n = 10). However, the reversibility observed with AFDX was much smaller to that observed after washout. The reversibility observed during a co-application of CCh with “AFDX + pirenzepine” (n = 8) was not different to that obtained during a co-application of CCh with “pirenzepine alone” (n = 7). These experiments were performed in STRC. *: p < 0.05 vs. reversibility after washout. B. Plot of the slope of neuronal AP firing during CCh addition in standard ACSF or in ACSF containing different mAChR antagonists as indicated. Note the slope of neuronal firing during application of CCh in a atropine-containing (n = 9) or a pirenzepine-containing (n = 7) ACSF is significantly smaller to that obtained during application of CCh in standard ACSF (n = 40). However, slope of neuronal firing during application of CCh in an AFDX-containing ACSF (n = 7) is not different to that obtained during application of CCh in standard ACSF (n = 40). These experiments were performed in STRC. The symbols represent: *: p < 0.05 vs. slope of neuronal firing during application of CCh in standard ACSF