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Figure 3 | BMC Neuroscience

Figure 3

From: In vitro study of uptake and synthesis of creatine and its precursors by cerebellar granule cells and astrocytes suggests some hypotheses on the physiopathology of the inherited disorders of creatine metabolism

Figure 3

Functional expression of GAMT and AGAT in rat astrocytes. Intracellular [13 C2,15N3]GAA (A) and [13C2,15N3]Cr (B) concentrations in function of [13C2,15N]Gly and[15 N2]Arg concentrations in culture medium, in three sets of cell samples (experiment 1, 2 and 3) are reported. To test the correlation among the intracellular concentration of labelled products and the precursor concentrations in culture medium, linear regression analysis was performed. Whereas there was a linear correlation for labelled GAA (p < 0.0022), no clear and consistent linear correlation for labelled Cr was found. Both graphs show that the cultures of experiment 3 yielded a lesser amount of GAA and Cr. Inspection of the cultures did not provide any obvious explanation for this result.

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BMC Neuroscience

ISSN: 1471-2202

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