Figure 6

Anatomical analysis of the mouse ON following X-ray irradiation. A-C, keratin staining showing A, intact basal lamina in control mice and B,C, disrupted basal lamina at 1 week post X-ray irradiation. Keratin (red), DAPI (blue). E-H, CD15 staining post X-ray irradiation of the ON. E, in control mice keratin (green) labeled horizontal basal cells and CD15 (red) labeled glandular cells in the lamina propria (small arrow) as well as the apical mucous layer (white arrows). F, 24 hours post-irradiation CD15-positive glandular cells (small arrows) were seen in the lamina propria but no longer in the apical mucous layer. G, 1 week post-irradiation CD15-positive glandular cells were seen traversing the basal lamina (small arrow). H, 2 weeks following irradiation CD15 labeling returned to the apical mucous layer (white arrow) and CD15-positive cells remained in the lamina propria (small arrow). D, βIIITubulin-positive olfactory neurons were seen in a disruption of the basal lamina 5 weeks post X-ray irradiation (white arrow). GFAP-positive olfactory ensheathing glia were seen surrounding axon bundles. βIIITubulin (red), GFAP (green), DAPI (blue). Dashed line indicates basal lamina. Scale bars: A-H, 50 μm.