Figure 2From: Neuroprotective effects of bis(7)-tacrine against glutamate-induced retinal ganglion cells damageGlutamate (Glu) induces a concentration- and time-dependent changes and bis(7)-tacrine is more potent than memantine in preventing the excitotoxicity of glutamate in the MTT assay. A, at 3 days in vitro, cultured RGCs were exposed to 1 μM bis(7)-tacrine for 2 h before the addition of glutamate at the different concentrations indicated. Cell viability was measured at 24 h by MTT assay. B, compared with normal neurons, RGCs were preincubated with or without 1 μM bis(7)-tacrine for 2 h and then exposed to 50 μM glutamate at different times indicated. Cell viability was measured at the indicated times. C, graph plotting percentage of neuron survival as a function of concentrations of bis(7)-tacrine(●)and memantine(○). RGCs were pre-treated with bis(7)-tacrine (0.001-1 μM) or memantine (0.01-10 μM) for 2 h before the addition of 50 μM glutamate. At 24 h after the challenges, cell viability was measured. All of the data, expressed as percentages of the corresponding control, were means ± SEM of three separate experiments. *P < 0.05, **P < 0.01 versus glutamate group in A and C or versus control at the same time in B. ++ P < 0.01 versus glutamate group at the same time in B.Back to article page