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Figure 5 | BMC Neuroscience

Figure 5

From: Cellular toxicity following application of adeno-associated viral vector-mediated RNA interference in the nervous system

Figure 5

AAV5 mediated knockdown of Npn-2 in DRG in vivo. AAV5 particles expressing GFP and a control shRNA (A, B, C) or shRNA directed against Npn-2 (D, E, F) were injected in the L4 and L5 DRG of 3 adult female wistar rats. GFP immunoreactivity (green: A, B, D, E) and Npn-2 mRNA (red: A, C, D, F) were visualized in the same section three weeks after AAV injection. A marked reduction in the percentage of Npn-2 expressing cells among the GFP positive cells was observed after shRNA expression: 16.5 ± 5.0% versus 37.2 ± 6.6% in control animals (G) (mean ± SEM, * p < 0.05). Scalebar A: 100 μm

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BMC Neuroscience

ISSN: 1471-2202

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