Figure 4
From: Signaling involved in neurite outgrowth of postnatally born subventricular zone neurons in vitro
Analysis of small GTPase activation in the SVZ/RMS neuronal culture. (A) Rac1 and (B) Cdc42 pull-down assays. Cultured cells were treated with inhibitors to PI3K, Akt1 and PKCζ and the amount of activated Rac1-GTP and Cdc42-GTP forms were measured by Western-blot. Relative band intensities are shown under each western-blot. Abbreviations: Ak - Akt1 inhibitor, C - control, LY - PI3K inhibitor LY294002, PZ - PKCζ inhibitor.