Figure 2From: A powerful transgenic tool for fate mapping and functional analysis of newly generated neuronsInduction of reporter gene expression by TAM injections at different developmental stages. a) Immunodetection of DCX expression in an E14.5 mouse embryo. Detection of β-gal activity at E15.5 in a DCX-CreERT2: Rosa26lacZ embryos injected with (b) TAM or (c) vehicle at E14.5. Arrow in (b) points to a labeled dorsal root ganglion. No β-gal signal could be detected in vehicle-injected embryos. Immunodetection of DCX (red), NeuN (blue) and EGFP (green) in the DG (d and e) and SVZ (f and g) of a 2 month-old DCX-CreERT2:CAG-CAT-EGFP mouse injected with TAM at E17.5. Arrowheads in (f) point to EGFP-expressing cells remaining at the ependymal lining. Immunodetection of DCX (red), NeuN (blue) and EGFP (green) in the adult OB (h and i) and DG (j and k) of DCX-CreERT2:CAG-CAT-EGFP mice 4 weeks after TAM injection in the adulthood. Arrow in (i) points at the co-labeling EGFP with DCX at the anterior end of the RMS. Scale bars = 1.7 mm in (a), 2 mm (b) and (c), 50 μm (k).Back to article page