Figure 2

Characterization of OlfDNCX. A. The PCR results from reverse transcription. Templates used for PCR reaction were the plasmid of the construct (plasmid), cDNA of olfactory turbinates in wild type (WT) and OlfDNCX mice. In OlfDNCX, OE- is the control that was processed identically as OE+ except missing SuperScript II in reverse transcription. B. Western analysis of protein homogenates of olfactory turbinates revealed a protein band around 120 kD in OlfDNCX, but not in WT, that was immunoreactive to antibodies against connexin 43 and β-galactosidase. C. A cartoon indicates the arrangement of epithelial cells in the olfactory epithelium. D and E. In situ hybridization in the olfactory epithelium. In situ hybridization using the antisense β-galactosidase riboprobe showed that the signal was localized to a band in the middle of the olfactory epithelial layer (D). The control (E) was processed identically as (D) except that sense β-galactosidase riboprobe was used. F and G. Confocal images displaying immunoreactivity for β-galactosidase (red) overlaid on Nomarski images. Immunostaining was observed in OlfDNCX (F), but not in WT (G). Arrowheads point to a, apical surface; and b, basal lamina. Bar = 20 μm.