Skip to main content
Figure 4 | BMC Neuroscience

Figure 4

From: Tricyclic pyrone compounds prevent aggregation and reverse cellular phenotypes caused by expression of mutant huntingtin protein in striatal neurons

Figure 4

Treatment with CP2 restores caveolin-related endocytosis inhibited by mhtt expression. A. Treatment with CP2 does not affect internalization of fluorescently labelled Tfn (red) in control or HD neurons. Images represent internalization of Tfn in live neurons 10 min after addition to the cells. Tfn internalization and localization were indistinguishable in untreated and CP2-pretreated control and HD cells. Data for 2 μM CP2 treatment is shown. Scale bar, 5 μm. B. Internalization of LacCer (green) is inhibited in striatal HD72 neurons (HD72, middle panel) comparing to control neurons. Treatment with 2 μM CP2 restores internalization of LacCer in HD72 neurons (HD72+2 μM CP2). Images of live cells in A and B were acquired using LSM 510 confocal microscope with 100× oil DIC lens (1.4 na). Scale bar, 5 μm. C. Quantification of Tfn and LacCer internalization in control and HD72 striatal neurons with and without CP2 treatment acquired in experiments shown in A and B. At least 10 cells were taken into analysis in each 3 independent experiments. *, p < 0.001.

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us