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Figure 2 | BMC Neuroscience

Figure 2

From: Effects of estrogens and bladder inflammation on mitogen-activated protein kinases in lumbosacral dorsal root ganglia from adult female rats

Figure 2

Adult female rat dorsal root ganglion (DRG) neurons cultured overnight in the absence of serum or added neurotrophic factors. a, Cultured DRG neuron immunostained for β-tubulin, showing extensive neurite growth. b, Two neurons showing immunoreactivity for ERα in the nucleus (arrows) and one neuron (bottom left) with no nuclear immunostaining. c, Neuron showing bright immunoreactivity for ERβ in the nucleus (arrow), moderate immunoreactivity in the cytoplasm and faint punctate staining in some neurites. d, e shows the same neuron, immunostained for ERα d) and nuclei labelled with DAPI (e); ERα-immunoreactivity is present in the neuronal nucleus (arrow) but not in the closely associated glia. Immunohistochemistry was performed in separate cultures from 3 rats. Calibration bar represents 20 μm (a-c), 10 μm (d, e).

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BMC Neuroscience

ISSN: 1471-2202

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