Fig. 4
From: Activation of the IL-17/TRAF6/NF-κB pathway is implicated in Aβ-induced neurotoxicity
Inhibition of IL-17 alleviates Aβ42-induced synaptic dysfunction. Normalized CA3-CA1 fEPSPs mean slope recorded from the CA1 dendritic region in hippocampal slices (A). Quantitative analysis of normalized fEPSPs slopes (B). N = 3, brain slices per mouse were recorded. TEM images (5000×magnification) showed the structure of synapses. Red arrows indicate the structure of the presynaptic and postsynaptic membranes and the synaptic cleft (C). Quantitative analyses of the number of synapses (D), N = 12, scale bar = 1 μm. Representative images (100×magnification) of dendritic spines of neurons from the Golgi-stained hippocampus (E). The average spine density (mean spine number per 10-mm dendrite segment) was measured in mice (F), N = 90, scale bar = 2 μm. PSD95 and SYN expression levels were detected by western blotting using specific antibodies, and actin was used as a loading control (G). Intensity analysis of SYN (H) and PSD95 levels (I). N = 3. Data are presented as the mean ± SD. p value significance is calculated from a one-way ANOVA test, *p < 0.05, **p < 0.01 and ***p < 0.001 vs the Aβ42 group. For western blotting original images, please see Additional file 1, and Adobe Photoshop software (2021) was used to cut the parts of interest from the original images