Fig. 2

Pathway enrichment analysis revealing the main biological processes of differentially expressed genes in the brain 2 h after cerebral ischemia. A Top 20 enriched biological processes; bar plot showing the number of differentially expressed genes of each altered biological process, sorted by increasing p-value from left to right. Each color indicates the corresponding transcriptomic dataset, and the purple color (integromics) corresponds to a subset of enriched pathways with combined evidence that were only detected by integrating the data of all groups. B Enrichment map of biological processes altered acutely after ischemic stroke. Nodes in the network represent biological processes, and similar biological processes with many common genes are connected. Nodes are colored according to the supporting omics datasets. Only pathways with 2 or more nodes were represented. C Venn diagram of the enriched biological processes in all studied groups. Diabetic: diabetic young male mice; Female: young female mice; Male: young male mice; Old: aged male mice