Table 1 PCR primers sequences for gene
Gene name | Primers |
|---|---|
hsa-Circ VRK1 | Forward:5′-TACCAACGAGCTGCAAAACC-3′ |
Reverse: 5′-TCACTCCCAAAGCGATCCAT-3′ | |
mmu-Circ VRK1 | Forward:5′-GGCGGACACAAATTCTTCCA-3′ |
Reverse: 5′-ACTCCCAAAGCGGTCCATTA-3′ | |
hsa-miR-17 | Forward:5′-TGCTTACAGTGCAGGTAG-3′ |
Reverse: 5′-GAACATGTCTGCGTATCTC-3′ | |
mmu-miR-17 | Forward:5′-AAGTGCTTACAGTGCAGGT-3′ |
Reverse: 5′-GAACATGTCTGCGTATCTC-3′ | |
(hsa and mmu) PTEN | Forward:5′-CATGTTGCAGCAATTCACTG-3′ |
Reverse: 5′-CTTGTGAAACAACAGTGCCA-3′ | |
(hsa and mmu)VEGF | Forward: 5′-TTGCCTTGCTGCTCTACCTCCA − 3′ |
Reverse: 5′-GATGGCAGTAGCTGCGCTGATA − 3′ | |
(hsa and mmu) U6 | Forward:5′-CTCGCTTCGGCAGCACATA-3′ |
Reverse: 5′-AACGCTTCACGAATTTGCG-3′ | |
(hsa and mmu) GAPDH | Forward: 5′-CATCACTGCCACCCAGAAGACTG-3′ |
Reverse: 5′-ATGCCAGTGAGCTTCCCGTTCAG-3′ |