Fig. 7

miR-17 inhibitor overturns the alleviating effects of Circ VRK1 siRNA on brain injury in mice. C57BL/6 mice with cerebral ischemia/reperfusion were injected with lentiviral vectors harboring Circ VRK1 si + miR-17 con, Circ VRK1 si + miR-17 inh, miR-17 inh + PTEN-NC or miR-17 inh + si-PTEN. A Circ VRK1, miR-17 and PTEN expression by PCR. B scoring of nerve damage in mice by Zea Longa scores. C brain tissue damage in mice by HE staining. D the area of cerebral infarction in mice examined by TTC staining. E IHC scores of CD34 in brain tissues. F Representative pictures of western blotting for VEGF protein expression in brain tissues, and all replicates are presented in the Additional file 1 (images of adequate length are not provided). Error bars represented the mean ± SD from 3 independent experiments. One-way (B, D, F) or two-way ANOVA (A) were used