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Fig. 6 | BMC Neuroscience

Fig. 6

From: Circ VRK1/microRNA-17/PTEN axis modulates the angiogenesis of human brain microvascular endothelial cells to affect injury induced by oxygen-glucose deprivation/reperfusion

Fig. 6

miR-17 inhibitor flattens the protection of Circ VRK1 siRNA on HBMVECs against OGD/R. HBMVECs were infected with lentiviral vectors harboring Circ VRK1 si + miR-17 con, Circ VRK1 si + miR-17 inh, miR-17 inh + PTEN-NC or miR-17 inh + si-PTEN and exposed to OGD/R. A Validation of lentiviral vector infection by qPCR. B proliferation of HBMVECs examined by colony formation assay. C apoptosis of HBMVECs examined by flow cytometry. D Migration of HBMVECs assessed by Transwell assay. E Tube formation of HBMVECs assessed by tube formation assay. F Representative pictures of western blotting for VEGF protein expression in HBMVECs, and all replicates are presented in the Additional file 1 (images of adequate length are not provided). Error bars represented the mean ± SD from 3 independent experiments. One-way (B–F) or two-way ANOVA (A) were used

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BMC Neuroscience

ISSN: 1471-2202

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