Fig. 3

Characterisation of INs by pseudotime trajectory and gene ontology analysis. A, B pseudotime analysis. A t-SNE plot showing the developmental trajectory of interneurons with inferred pseudotime trajectories by Monocle3 (inset reproduced from Fig. 1D for reference). Each dot represents an individual cell in IN 1–8 and coloured according to it’s position on the pseudotime trajectory from 0.00 (least differentiated—dark blue) to 1.00 (most differentiated—yellow). B Density plot estimating the distribution of numbers of cells in each interneuron cluster (IN 1–8) along the pseudotime trajectory. C–G Gene ontology (GO) analysis. GO analysis in IN1,3,5,8 (orange in C) versus other INs (green in C) reveals enrichment of GO terms associated with D synaptic maturation and plasticity and axon extension and guidance. E gradient plots of MEF2C, ADCY1, and SYT4 showing that these transcripts are expressed in a gradient across INs with highest expression in IN5,8. F, G Gene ontology (GO) analysis in IN5,8 (orange in F) versus other INs (green in F) reveals enrichment of GO terms associated with G synaptic maturation and plasticity