Fig. 5From: Effects of low-doses of methamphetamine on d-fenfluramine-induced head-twitch response (HTR) in mice during ageing and c-fos expression in the prefrontal cortexDouble-labeled immunofluorescent staining for c-fos (red; a), DAPI (blue; b) and the merged image (c) in mice PFC. c-fos immunoreactivity was counted when the cell nucleus was round or oval, completely filled, and double-labeled with DAPI, scale bars = 10 μmBack to article page