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Fig. 4 | BMC Neuroscience

Fig. 4

From: Effects of low-doses of methamphetamine on d-fenfluramine-induced head-twitch response (HTR) in mice during ageing and c-fos expression in the prefrontal cortex

Fig. 4

Pretreatment with either the selective 5-HT1A receptor antagonist WAY 100635 (0.25 mg/kg, i.p., a), or the selective ɑ2-adrenergic receptor antagonist RS 79948 (0.1 mg/kg, i.p., b), reversed the inhibitory action of MA (1 mg/kg, i.p.) on d-fenfluramine-induced (5 mg/kg, i.p.) HTR in 20- and 30-day old mice. Relative to the corresponding age-matched Vehicle + Vehicle + d-fenfluramine pretreated group, MA pretreatment (i.e. MA + Vehicle + d-fenfluramine treatment group) significantly reduced the frequency of d-fenfluramine-induced HTR in 20- (*p = 0.0342) and 30-day old mice (****p < 0.0001; a, b); whereas WAY 100635 pretreatment (i.e. Vehicle + WAY 100635 + d-fenfluramine treatment group) significantly potentiated the frequency of d-fenfluramine-induced HTR in 30-day (####p < 0.0001, a) but not in 20-day old mice. Relative to the corresponding MA + Vehicle + d-fenfluramine treatment group, inclusion of WAY 100635 (i.e. the MA + WAY 100635 + d-fenfluramine treatment group) only significantly reversed the inhibitory effect of MA on DOI-induced HTR in 30-day old mice (****p < 0.0001, a); whereas inclusion of RS 79948 (i.e. the MA + RS 79948 + d-fenfluramine treatment group) significantly reversed the inhibitory effect of MA on DOI-induced HTR in both 20 (**p = 0.0082, b)- and 30 (****p < 0.0001, b)-day old mice. Two-way ANOVA followed by Dunnett's test. n = 6 − 9 in each group. Data are presented as means ± SEM

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