Fig. 6From: SUFI: an automated approach to spectral unmixing of fluorescent multiplex images captured in mouse and post-mortem human brain tissuesSpectral unmixing of immunofluorescence data from post-mortem Alzheimer’s disease brain tissue. The cell nuclei are stained with DAPI, Abeta protein is labelled with AF488, pTau protein is labeled with AF555, MAP2 protein is labeled with AF633. Images shown are 2D maximum intensity projections of 3D z-stacks. A–E Linear unmixing results using ZEN software. A′–E′ Spectral unmixing results using FCLSU algorithm. A″–E″ Spectral unmixing results using ELMM algorithm. A‴–E‴ Spectral unmixing results using GELMM algorithmBack to article page