Fig. 3

Spectral unmixing of smFISH data from cultured mouse neurons. Neuronal nuclei stained with DAPI, Fos transcripts labeled with Opal520, Bdnf exon 1 transcripts labeled with Opal570, Bdnf exon 4 transcripts labeled with Opal620, and Arc transcripts labeled with Opal690. Images shown are 2D maximum intensity projections of 3D z-stacks. A–E Linear unmixing results using ZEN software. A′–E′ Spectral unmixing results using FCLSU algorithm. A″–E″ Spectral unmixing results using ELMM algorithm. A‴–E‴ Spectral unmixing results using GELMM algorithm