Fig. 4

Sul-F treatment significantly attenuated the I/R-induced ER stress (n = 6). A At 24 h post-reperfusion, levels of p-PERK, p-eIF2α, ATF4 were evaluated with western blot. B The histogram showed quantification of images in bar diagram. The relative value of band gray was measured with Image J (1.49 V) and normalized to that of β-actin. C The mRNA levels of PERK, eIF2α and ATF4 were evaluated with qRT-PCR. D Representative western blot results of p-IRE1, Caspase12 and Caspase3 were shown. E The histogram showed quantification of images in bar diagram. The relative value of band gray was measured with Image J (1.49 V) and normalized to that of β-actin. F The mRNA levels of IRE1, Caspase12 and Caspase3 were evaluated with qRT-PCR. Data were expressed as the mean ± SEM from different assays. ^**P < 0.01 compared with Sham group, ^△P < 0.05 and ^△△P < 0.01 compared with the MCAO group, ^#P < 0.05 and ^##P < 0.01 compared with the EDA group, ^aP < 0.05 and ^aaP < 0.01 compared with the Sul-F-H group