Fig. 3

Sul-F treatment significantly alleviated the I/R-induced apoptosis in the penumbra of brain after 24 h reperfusion. A TdT-mediated dUTP Nick-End Labeling (TUNEL) staining of I/R-induced apoptosis in penumbra, which was imaged at 100 × . TUNEL cells (red) and the nuclei (DAPI, blue). B The I/R-induced apoptosis presented as ratio = (TUNEL positive cells) / (DAPI cells) × 100% (n = 5). C Representative western blot analysis of CHOP, Bax (pro-apoptotic protein) and Bcl-2 (anti-apoptotic protein) in the penumbra of brain tissue (n = 6). D Histogram showing quantification of images in C (n = 6). The results were normalized to β-actin expression. E Histogram showing quantification of qRT-PCR (n = 6). Data were expressed as the mean ± SEM from different assays. ^**P < 0.01 compared with the Sham group, ^△P < 0.05 and ^△△P < 0.01 compared with the MCAO group, ^#P < 0.05 and ^##P < 0.01 compared with the EDA group, ^aaP < 0.01 compared with the Sul-F-H group