Fig. 9

Group-based comparison of three threshold finding methods. On the example of the \(Nacc1^{em1(IMPC)Hmgu}\) mouse line, the figure shows an end-to-end comparison of the three threshold finding methods for the use case of identifying candidate genes with hearing function involvement. For each method (manual: top left, NN: top right, SLR: bottom left), stimulus-specific hearing thresholds are compiled to median hearing curves of mutants (triangles and solid lines), same-day reference controls (squares and dashed lines) and “all controls” (circles and dotted lines) and shown as symbols, which are connected for the non-click stimuli. The ribbon shows the [5;95] percentile range of all controls. Numbers of mice in each group are shown in the legend. On the y-axis, hearing threshold is given in 20 dB ticks. The x-axis shows the stimulus. Whiskers indicate inter-quartile ranges. Each method-specific plot allows comparison of mutant vs. control hearing curves with thresholds based on that method. The bottom-right figure shows mutant-only median hearing curves and [5;95] percentile ranges of the three methods (manual: blue, solid line and circle; NN: green, dotted line and triangle; SLR: red, dashed line and star) to allow method comparison