Fig. 11
From: CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data
Pharmacological blockade of synaptic transmission illustrates the specificity of CaPTure: A graph showing normalized calcium activity from a sample baseline field and the estimated background (0.5), Scale 0–3.1088. Scale bar indicates: 60 s on x-axis and 1.5 units of normalized mean fluorescence intensity on y-axis. B Graph showing normalized calcium activity of the same field following TTX treatment, with thresholds from baseline activity to minimize contribution of background noise to detected signal. C Graph showing normalized calcium activity of the same pharmacology sample from B with the respective intensity scale (y-axis) and background estimated from the TTX-treated sample, Scale 0–0.397. Scale bar indicates: 60 s on x-axis and 0.02 units of normalized mean fluorescence intensity on y-axis. D Boxplots showing the calcium activity of neurons from baseline and TTX-treated fields