Skip to main content
Fig. 4 | BMC Neuroscience

Fig. 4

From: Mechanism of LSD1 in oxygen-glucose deprivation/reoxygenation-induced pyroptosis of retinal ganglion cells via the miR-21-5p/NLRP12 axis

Fig. 4

Silencing miR-21-5p averts the repressive effect of silencing LSD1 on OGD/R-induced pyroptosis of RGCs. RGC-5 cells were infected with miR-21-5p inhibitor lentivirus (miR-in), and inhibitor NC lentivirus (miR-NC) was used as the negative control. A: The expression levels of miR-21-5p in RGC-5 cells were examined via RT-qPCR; B: RGC-5 cell viability was detected via the CCK-8 method; C: The concentrations of IL-1β and IL-18 were examined via ELISA; D: The mRNA levels of Caspase 1 in RGC-5 cells were determined via RT-qPCR; E: The protein levels of NLRP3, GSDMD-N, and cleaved-Caspase1 in RGC-5 cells were examined via Western blot analysis. Each cell experiment was performed in triplicate; measurement data were presented as mean ± standard deviation (SD); data in panel A were analyzed via the t-test; data in panels B and D were analyzed via one-way ANOVA and data in panels C and E were analyzed via two-way ANOVA, followed by Tukey’s multiple comparison test; *p < 0.05

Back to article page