Skip to main content
Fig. 2 | BMC Neuroscience

Fig. 2

From: Mechanism of LSD1 in oxygen-glucose deprivation/reoxygenation-induced pyroptosis of retinal ganglion cells via the miR-21-5p/NLRP12 axis

Fig. 2

Silencing LSD1 represses OGD/R-induced pyroptosis of RGCs. RGC-5 cells were infected with shRNA-LSD1 lentivirus (sh-LSD1), with shRNA-NC lentivirus (sh-NC) as the negative control. A: The protein levels of LSD1, NLRP3, GSDMD-N, and cleaved-Caspase1 in RGC-5 cells were examined via Western blot analysis; B: The mRNA levels of LSD1 and Caspase 1 in RGC-5 cells were determined via RT-qPCR; C: The concentrations of IL-1β and IL-18 were examined via ELISA; D: RGC-5 cell viability was detected via the CCK-8 method. Each cell experiment was performed in triplicate; measurement data were presented as mean ± standard deviation (SD); data in panel B (left) were analyzed via the t-test; data in panels B (right) and D were analyzed via one-way ANOVA and data in panels A and C were verified via two-way ANOVA, followed by Tukey’s multiple comparison test; *p < 0.05

Back to article page