Fig. 6

Let-7b-5p antagomir abolished rTMS effects on HMGA2/NF-κB pathway and microglia polarization. a Immunofluorescence staining to detect the successful transfection of Cy5-let-7b-5p antagomir (red), nucleus was in blue. b Real-time PCR for the detection of let-7b-5p level after NC antagomir or let-7b-5p antagomir transfection in BV2 cells (n = 3. ***P < 0.001 versus NC antagomir group). c, d Western blots and quantification of HMGA2/ NF-κB pathway associated proteins and microglia polarization markers in NC antagomir or let-7b-5p antagomir transfected OGD/R injured BV2 cells with or without rTMS treatment. n = 3. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 versus OGD/R group; ^#P < 0.05, ^##P < 0.01 versus OGD/R + rTMS group. Scale bar: 5 μm