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Fig. 4 | BMC Neuroscience

Fig. 4

From: High-frequency repetitive transcranial magnetic stimulation (rTMS) protects against ischemic stroke by inhibiting M1 microglia polarization through let-7b-5p/HMGA2/NF-κB signaling pathway

Fig. 4

The 10 Hz rTMS increased microglial expression of let-7b-5p after ischemia–reperfusion and OGD/R injury. a Heatmap of the microRNAs with at least two-fold change and CT value > 200 in expression in cerebral peri-infarct tissues. b Real-time PCR for validation of miRNAs significantly increased by the 10 Hz rTMS treatment in the cerebral peri-infarct tissues of MCAO rats. c Real-time PCR for validation of the five miRNAs in OGD/R BV2 cells. d Combined FISH and IF for let-7b-5p (red) and Iba-1 (green) in the peri-infarct region of MCAO rats. e FISH for the staining of let-7b-5p in OGD/R BV2 cells. n = 3. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 versus Sham or Ctrl group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus MCAO or OGD/R group. Scale bar: 10 μm

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