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Fig. 3 | BMC Neuroscience

Fig. 3

From: High-frequency repetitive transcranial magnetic stimulation (rTMS) protects against ischemic stroke by inhibiting M1 microglia polarization through let-7b-5p/HMGA2/NF-κB signaling pathway

Fig. 3

The 10 Hz rTMS alleviated neurotoxicity of microglia after OGD/R injury. a TNF-α concentration and b IL-10 concentration in Ctrl MCM, OGD/R MCM and OGD/R + rTMS MCM. c, d Neurons were co-cultured with Ctrl MCM, OGD/R MCM and OGD/R + rTMS MCM for 48 h, PI/Hoechst staining was applied to detect vitality of neurons. Dead neurons were positively stained with red PI (d) and dead neurons were quantified by Image J (c). n = 5. Data are expressed as mean ± SD; **P < 0.01, ***P < 0.001. MCM, microglia culture media. Scale bar: 10 μm

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