Fig. 6

Cell-specific location of caspase-1 in the lumbar spinal cord. Representative images of double immunofluorescence staining for caspase-1 with NeuN, GFAP, or Iba1 are shown. At 60 d, caspase-1 was mainly expressed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a. Increased co-localization of caspase-1 with activated GFAP⁺ astrocytes and Iba1⁺ microglia in ALS mice were observed from 95 to 122 d b, c, d. Arrows indicate double-labelled cells. Scale bar 50 μm