Fig. 5

Cell-specific location of NLRP3 in the lumbar spinal cord. Representative images of double immunofluorescence staining for NLRP3 with NeuN, GFAP, or Iba1 are shown. At 60 d, NLRP3 was mainly expressed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a. Dramatic co-localization with neurons and activated GFAP⁺ astrocytes in ALS mice was observed at 95 d b. Increased NLRP3 expression was observed in GFAP⁺ and Iba1⁺ cells in the ALS spinal cord c, d. Arrows indicate double-labelled cells. Scale bar 50 μm