Fig. 2

Expression profiling of the changes in mRNAs in SARS-CoV-2-infected SK-N-SH cells. A Heat map showing unsupervised clustering of significantly expressed mRNAs from SK-N-SH cells in the SARS-CoV-2 infection groups compared with in the control groups. The expression profiles are displayed with three samples in each group. Red represents high relative expression and blue represents low relative expression. B Volcano plot of the upregulated and downregulated mRNAs from SK-N-SH cells in the SARS-CoV-2 infection group compared with in the control group. Increases of ≥ 2-fold or decreases to ≤ 0.5-fold (p < 0.05) were considered statistically significant. C, D The relative transcription level of 20 mRNAs, including 10 downregulated and 10 upregulated mRNAs, were examined by RT-qPCR in SK-N-SH cells treated with or without SARS-CoV-2 for 72 h. GAPDH was used as the internal reference. Data were presented as the mean ± standard deviation (SD) from three independent experiments. **p < 0.01, ***p < 0.001