Fig. 3

Hippocampal Sirt1 knockdown caused cognitive impairment in middle-aged mice. A Percentage of time spent by mice in the center zone during the OFT. B Representative autonomous trajectory maps of mice in OFT. Green color indicates the defined center zone and the rest is defined peripheral zone. C The swimming speed of mice in MWM with visual platform before the learning phase. D Representative swimming paths of mice in the probe phase. E Escape latency of mice during the learning phase. F Time spent in target zone and (G) the number of platform crossings of mice in the probe phase. n = 12. ^*p < 0.05 and ^**p < 0.01 represent comparison between the control group and the shSirt1 group. ^#p < 0.05 represents comparison between the sham group and the shSirt1 group