Fig. 5

Analysis of immunohistochemical staining for MBP in coronal brain slices of vehicle- and T4-treated rat pups. a Representative examples of MBP protein staining after treatment with thyroxine (T4) or vehicle at PND5–11. b Quantification of the MBP signal optical density at PND5 and PND7–PND11. Six sagittal adjacent brain sections containing corpus callosum rostral to the hippocampus from at least two animals per group were used for this analysis. T4 treatment significantly increased MBP expression levels at PND 9 and 11. Data are presented as the mean ± SEM (n = 6). For the data quantification analysis, the regions of T4 vs. vehicle were compared to using the Student’s t-test and the correction for multiple comparisons was done by controlling for the false discovery rate (FDR; set at q = 0.1) by using the Benjamini–Krieger–Yekutieli procedure implemented in Prism 9. Data are presented as the mean ± SEM. Differences were considered statistically significant if adjusted P < 0.05. Asterisk (*) denotes P values of T4 compared to vehicle for each postnatal day,*P < 0.05 (Student’s t-test). Black and white bars denote T4-treated and vehicle-treated pups, respectively