Fig. 2

BLAST RNA-Seq RPKM values quantifying Fmr1 sequences in the rat forebrain. Box plots of RPKM values for BLAST searches with (A) Fmr1 coding sequence from exon 1 through 11 [1–11]; or sequences spanning junctions between (B) exons 10 and 11, (C) 16 and 17B, (D) 13 and 14, (E) 14 and the summation of data for the three exon 15 sites. F RPKM ratios of 13–15A, 13–15B or 13–15C junction by exon 10 and 11 junction sequence reads. Significant Dunn´s post-hoc test results are indicated on graphs by lines with asterisks according to P values for comparisons to E14 RPKM values (*P < 0.05; **P < 0.01; ***P < 0.001). Other significant comparisons between embryonic ages or involving P3 are shown below each graph (A–E). N = 4 for each age group, except E14 and P7 (N = 3), and E17 (N = 6)