Fig. 1

Cellular distribution of CCL2-ir in cortex and CC of saline and LPS injected mice. A, B, CCL2-ir positive structures and neuron like cells scatter in the cortex of saline (A) and LPS (B) injected mice, respectively. The former is generally situated in the area surrounding the blood vessel like profiles (arrows and insets), and the latter is mostly located in I ~ III of the cortex layers (arrowheads and insets). The vasculature with negative perivascular labeling is also observed (opened arrowhead and inset in B). C, D, the CC in saline injected mice, showing majority of CCL2-ir labeling are located in lateral CC between Cg and LV (aligned arrows), and these labeling are commonly surrounding or laying against vasculature like profiles, like what is seen in neighbor cortex (inset in C). Occasionally, the neuron-like CCL2-ir positive cell is encountered in the CC (inset in D). E−H Rostral (E, F), middle (G) and caudal (H) plane of the CC in LPS treated mice, in which a large number of CCL2-ir positive cashew shape elements were viewed scattering in entire CC WM tract in coronal planes, and the opened arrows point to the typical or larger ones. These cashew like CCL2-ir labeling seems be inside of microvasculature, and more of them are distributed in the CC (E– H, opened arrows) than in the cortex. Cg, cingulate cortex; Ctx, cortex; LV, lateral ventricle; SF, sagittal fissure. Scare bar = 100 µm in all A– H