Skip to main content
Fig. 2 | BMC Neuroscience

Fig. 2

From: Impacts of autofluorescence on fluorescence based techniques to study microglia

Fig. 2

PE-conjugated antibody outperforms FITC and APC conjugated antibodies in detecting microglia LAG3. A Distribution of fluorescence intensities from FITC conjugated isotype control antibody labeled microglia with the previous blocking by mouse IgG, CD16/CD32/CD16.2 antibodies, or the combination of mouse IgG and CD16/CD32/CD16.2 antibodies. B Distribution of fluorescence intensities from nontreated microglia and isotype control antibody treated microglia. C Distribution of fluorescence intensities from nontreated microglia and FITC, PE, and APC conjugated LAG-3 antibodies and their respective isotype control antibodies treated microglia. D Median fluorescence intensity (MFI) of microglia as treated in (C). E Comparison of MFI LAG-3/MFI IgG of FITC, PE, and APC conjugated LAG-3 antibodies (n = 4 mice). Bars represent the means ± standard deviation (SD). Comparisons were made by one-way ANOVA test with Tukey’s post hoc multiple comparisons test. *P < 0.05; **P < 0.01; NS, not significantly different

Back to article page