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Fig. 6 | BMC Neuroscience

Fig. 6

From: Paeonol regulates NLRP3 inflammasomes and pyroptosis to alleviate spinal cord injury of rat

Fig. 6

Paeonol suppresses the pyroptosis and inflammatory responses in BV-2 cells. A The mRNA expression of caspase1 and NLRP3 in BV-2 cells was detected by qRT-PCR. ***P < 0.001 vs. the control group. B The western blot assay images (20 μg/lane) for the levels of ASC, NLRP3, active caspase 1, and N-GSDMD in BV-2 cells. C The protein level of ASC in BV-2 cells was measured by western blot assay. D The protein level of NLRP3 in BV-2 cells was measured by western blot assay. E The protein level of active caspase 1 in BV-2 cells was measured by western blot assay. F The protein level of N-GSDMD in BV-2 cells was measured by western blot assay. G The level of IL-1β i in BV-2 cells was measured by ELISA. H The level of IL-18 in BV-2 cells was measured by ELISA. I The level of TNF-α in BV-2 cells was measured by ELISA. ***P < 0.001 vs. the control group. ##P < 0.01, ###P < 0.001 vs. the LPS/ATP group

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