Table 1 Primary and secondary antibody information for western analysis
From: Repair-related molecular changes during recovery phase of ischemic stroke in female rats
Antibodies | Dilution | Supplier (catalogue no.) |
|---|---|---|
Primary antibodies | ||
 Akt | 1:1000 | Cell signaling (9272S) |
 p-Akt (Ser473) | 1:1000 | Cell signaling (4058S) |
 p-ERK1/2 | 1:2000 | Cell signaling (9101) |
 t-ERK1/2 | 1:4000 | Cell signaling (9107) |
 IL-10 | 1:1000 | Abcam (ab25073) |
 Nestin | 1:1000 | Abcam (ab6142) |
 NeuN | 1:10,000 | Abcam (ab104224) |
 TGF-β | 1:250 | Abcam (ab66043) |
 Tie-2 | 1:200 | SantaCruz (sc-9026) |
Secondary antibodies | ||
 Anti-rabbit IgG:peroxidase | 1:2000 | Cell signaling (7074) |
 Anti-mouse IgG:peroxidase | 1:2000 | Cell signaling (7076) |