Fig. 6

Intestine-specific RORA deficiency mimics or worsens maternal diabetes-mediated RORA suppression and oxidative stress in IEC. Dams in either the control (CTL) or diabetes (STZ) groups were crossbred with either wild type (WT) or intestinal epithelial-specific RORA knockout (RORA^−/−) mice, and the IEC cells were isolated from subsequent male offspring for biomedical analysis. a mRNA levels by qPCR, n = 4. b Quantitation of protein levels, n = 5. c Representative pictures of western blotting for b, and full-length blots are presented in Additional file 1: Fig. S1c. d ROS formation, n = 5. e 3-Nitrotyrosine formation, n = 5. f SOD2 activity, n = 5. g, h The IEC were isolated for 8-oxo-dG immunostaining; g representative pictures for 8-oxo-dG staining (green) and DAPI staining for nuclei (blue); h quantitation of 8-oxox-dG staining, n = 5; The two-way ANOVA analysis was performed to determine statistical significance of different groups. ***P < 0.0001; **P < 0.001; *P < 0.01; N/S, no significance. Data were expressed as mean ± SD