Fig. 1

RORA agonist and SOD mimetic reverse maternal diabetes-mediated RORA suppression and oxidative stress in the amygdala. Dams were divided into the following treatment groups: control (CTL/VEH), diabetes (STZ/VEH), diabetes plus SOD mimetic MnTBAP (STZ/MnTBAP), and diabetes plus RORA agonist SR1078 (STZ/SR1078) during pregnancy, and the amygdala neurons or tissues were isolated from male offspring for biomedical analysis. a mRNA levels by qPCR, n = 4. b Quantitation of protein levels, n = 5. c Representative pictures of western blotting for b, and full-length blots are presented in Additional file 1: Fig. S1a. d ROS formation, n = 5. e 3-Nitrotyrosine formation, n = 5. f SOD2 activity, n = 5. g, h The amygdala neurons were isolated on embryonic day (E18) from the above treatment for immunostaining. g Quantitation of 8-oxox-dG staining, n = 5. h Representative pictures for 8-oxo-dG staining (green) and DAPI staining for nuclei (blue). The one-way ANOVA analysis was performed to determine statistical significance of different groups. *P < 0.05, vs. CTL/VEH group; ^¶P < 0.05, vs. STZ/VEH group. Data were expressed as mean ± SD