Fig. 6

Effect of mitoxantrone with/without exercise on A BDNF in the CSF and B–G Foxp3 in the brain stem of EAE rats. B Number of Foxp3 positive cells in the brain stem of exercised and sedentary rats. Immunostaining of Foxp3 showing absence of positively stained lymphocytes in C normal control rats. Sections of EAE untreated rats show positively stained perivascular lymphocytes (arrows) in the active plaques of D sedentary/E exercised groups, whereas treatment with mitoxantrone shows few positively stained lymphocytes in the active plaques of F sedentary/G exercised groups. Values are presented as mean ± S.D (n = 6/group). Comparisons inside the same group was done using one-way ANOVA followed by Tukey's Multiple Comparison Test. Comparison between exercised and sedentary groups was done using two-way ANOVA followed by Bonferroni Correction Test (p < 0.05). As compared to CN_SED (*), CN_EX ($), EAE_SED (#), EAE_EX (%) and exercised vs sedentary rats (ψ). EAE experimental autoimmune encephalomyelitis; groups of CN_SED sedentary control, CN_EX exercised control, EAE_SED sedentary untreated EAE, EAE_EX exercised untreated EAE, MT_SED sedentary mitoxantrone, MT_EX exercised mitoxantrone