Fig. 1
Timelines for injections, HTR observation, and perfusion. a and b Corresponding vehicle (i.p.), or varying doses of the EMD 281014 (0.01, 0.05, 0.1 mg/kg, i.p.), or MA (1, 2.5, 5 mg/kg, i.p.), were injected to different groups of mice (20-, 30- or 60-day old) 30 min prior to DOI (1 mg/kg, i.p.) injection. The frequency of HTRs were observed for 30 min post DOI injection. Based on behavioral data, 30-day-old mice were selected to perform the immuno-histochemistry studies. Thus, after injections and HTR observation, mice were perfused at 120 min (a, b). For behavioral interaction studies, different groups of mice (20-, 30, and 60-day old) received either a single dose of MA (5 mg/kg, i.p.) or its vehicle at 0 min, and at 20 min received either the 5-HT1A receptor antagonist WAY 100635 (0.25 mg/kg, i.p.), or the ɑ2-adrenergic-receptor antagonist RS 79948 (0.1 mg/kg, i.p.), or corresponding vehicle. At 30 min DOI (1 mg/kg, i.p.) was injected (c, d) and the HTR frequency were observed for 30 min post DOI injection